MersV1, Main, Exploration, bibRecord, 004523

Dipentafluorophenyl carbonate--a reagent for the synthesis of oligonucleotides and their conjugates.

Identifieur interne : 004523 ( Main/Exploration ); précédent : 004522; suivant : 004524

Dipentafluorophenyl carbonate--a reagent for the synthesis of oligonucleotides and their conjugates.

Auteurs : V A Efimov [Russie] ; A L Kalinkina ; O G Chakhmakhcheva

Source :

Nucleic acids research [ 0305-1048 ] ; 1993.

RBID : pubmed:8265346

Descripteurs français

KwdFr :
- Données de séquences moléculaires, Fluorocarbones (), Oligodésoxyribonucléotides (), Oligodésoxyribonucléotides (synthèse chimique), Phosphonates (), Séquence nucléotidique.
MESH :
- synthèse chimique : Oligodésoxyribonucléotides.
- Données de séquences moléculaires, Fluorocarbones, Oligodésoxyribonucléotides, Phosphonates, Séquence nucléotidique.

English descriptors

KwdEn :
- Base Sequence, Fluorocarbons (chemistry), Molecular Sequence Data, Oligodeoxyribonucleotides (chemical synthesis), Oligodeoxyribonucleotides (chemistry), Organophosphonates (chemistry).
MESH :
- chemical , chemical synthesis : Oligodeoxyribonucleotides.
- chemical , chemistry : Fluorocarbons, Oligodeoxyribonucleotides, Organophosphonates.
- Base Sequence, Molecular Sequence Data.

Abstract

Dipentafluorophenyl carbonate has been successfully used as condensing agent for the internucleotide bond formation in the synthesis of oligonucleotides via H-phosphonate approach. The mechanism of a nucleotide component activation with this reagent has been investigated with the help of 31P NMR spectroscopy. It was shown that preactivation of deoxynucleoside H-phosphonate with dipentafluorophenyl carbonate has no influence on the efficiency of the synthesis. This reagent is highly reactive, nonhygroscopic and stable on storage at room temperature. The effectiveness of dipentafluorophenyl carbonate in the oligonucleotide chemistry has been demonstrated in the solid-phase synthesis of 10-50-mers on 0.2, 1 and 10 mumol scales. The use of this reagent for the derivatisation of polymer supports as well as for the synthesis of oligonucleotide conjugates with polyethylene glycol and a lipid is described.

DOI: 10.1093/nar/21.23.5337
PubMed: 8265346

Affiliations:

Le document en format XML

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<front><div type="abstract" xml:lang="en">Dipentafluorophenyl carbonate has been successfully used as condensing agent for the internucleotide bond formation in the synthesis of oligonucleotides via H-phosphonate approach. The mechanism of a nucleotide component activation with this reagent has been investigated with the help of 31P NMR spectroscopy. It was shown that preactivation of deoxynucleoside H-phosphonate with dipentafluorophenyl carbonate has no influence on the efficiency of the synthesis. This reagent is highly reactive, nonhygroscopic and stable on storage at room temperature. The effectiveness of dipentafluorophenyl carbonate in the oligonucleotide chemistry has been demonstrated in the solid-phase synthesis of 10-50-mers on 0.2, 1 and 10 mumol scales. The use of this reagent for the derivatisation of polymer supports as well as for the synthesis of oligonucleotide conjugates with polyethylene glycol and a lipid is described.</div>
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Dipentafluorophenyl carbonate--a reagent for the synthesis of oligonucleotides and their conjugates.

Dipentafluorophenyl carbonate--a reagent for the synthesis of oligonucleotides and their conjugates.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki

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